PRKDC (phospho T2609) polyclonal antibody-抗体-抗体-生物在线
亚诺法生技股份有限公司（Abnova)
PRKDC (phospho T2609) polyclonal antibody

PRKDC (phospho T2609) polyclonal antibody

商家询价

产品名称： PRKDC (phospho T2609) polyclonal antibody

英文名称： PRKDC (phospho T2609) polyclonal antibody

产品编号： PAB10324

产品价格： null

产品产地： 台湾

品牌商标： Abnova

更新时间： null

使用范围：

亚诺法生技股份有限公司（Abnova)
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of PRKDC.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding T2609 of human PRKDC.
  • Host:
  • Rabbit
  • Reactivity:
  • Chicken, Chimpanzee, Dog, Horse, Human, Rat
  • Specificity:
  • This phospho-specific polyclonal antibody reacts with phosphorylated pT2609 of human DNA PKcs. Reactivity with non-phosphorylated human DNA PKcs is minimal.
  • Form:
  • Liquid
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:300000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using PRKDC (phospho T2609) polyclonal antibody (Cat # PAB10324) shows detection of a 460 KDa band corresponding to human PRKDC in various preparations.
    Lane 1 : Fus1 untreated, Lane 2 : Fus1 IR (20Gy, 4h), Lane 3 : Fus1 PRKDC inhibitor + IR, Lane 4 : MO59J (DNAPK-) untreated, Lane 5 : MO59J IR, Lane 6 : Fus1 untreated, Lane 7 : Fus1 IR (20Gy, 4h), Lane 8 : Fus1 DNAPK inhibitor + IR, Lane 9 : MO59J untreated, Lane 10 : MO59J IR.
    Lanes 1~5 are nuclear extract whereas lanes 6~10 are whole cell lysates.
    MO59J is a cell line that lacks PRKDC.
    FUS1 is the matched cell line complemented with a chromosomal fragment containing the PRKDC gene.
    Approximately 20 $micro;g of lysate was run on SDS-PAGE and transferred onto nitrocellulose, followed by reaction with a 1 : 1,000 dilution of PRKDC (phospho T2609) polyclonal antibody (Cat # PAB10324).
    Detection occurred using a 1 : 5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature.
    A che
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 5591
  • Protein Accession#:
  • P78527;NP_008835
  • Gene Name:
  • PRKDC
  • Gene Alias:
  • DNA-PKcs,DNAPK,DNPK1,HYRC,HYRC1,XRCC7,p350
  • Gene Description:
  • protein kinase, DNA-activated, catalytic polypeptide
  • Gene Summary:
  • The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties.[supplied by OMIM
  • Other Designations:
  • hyper-radiosensitivity of murine scid mutation, complementing 1

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