硫氧还蛋白抗体
产品名称： 硫氧还蛋白抗体
英文名称： Thioredoxin
产品编号： 0458
产品价格： null
产品产地： 上海
品牌商标： 雅吉
更新时间： null
使用范围： WB ELISA IHC-P IHC-F IF
上海雅吉生物科技有限公司
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| 中文名称 | 硫氧还蛋白抗体 |
| 别 名 | ADF; Surface associated sulphydryl protein; Thioredoxin-1; Thioredoxin 1; Thioredoxin1; TXN protein; ADF; ATL derived factor; DKFZp686B1993; MGC61975; SASP; Surface associated sulphydryl protein; Surface-associated sulphydryl protein; Thioredoxin; TRDX; TRX 1; TRX; TRX1; TXN; TXN; ATL derived factor; ATL-derived factor; THIO_HUMAN; zgc:92903. |
| 研究领域 | 肿瘤 细胞生物 免疫学 信号转导 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, fusion protein (predicted: Dog, Pig, Cow, Horse, Rabbit, ) |
| 产品应用 | WB=1:1000-5000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 12kDa |
| 细胞定位 | 细胞核 细胞浆 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Thioredoxin:51-105/105 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] Function: Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity. Subunit: Homodimer; disulfide-linked. Interacts with TXNIP through the redox-active site. Interacts with MAP3K5 and CASP3. In case of infection, interacts with S.typhimurium protein slrP. Interacts with APEX1; the interaction stimulates the FOS/JUN AP-1 DNA-binding activity in a redox-dependent manner. Subcellular Location: Nucleus. Cytoplasm. Secreted. Note=Secreted by a leaderless secretory pathway. Predominantly in the cytoplasm in non irradiated cells. Radiation induces translocation of TRX from the cytoplasm to the nucleus. Similarity: Belongs to the thioredoxin family. Contains 1 thioredoxin domain. SWISS: P10599 Gene ID: 7295 Database links: Entrez Gene: 7295 Human Entrez Gene: 22166 Mouse Entrez Gene: 116484 Rat Omim: 187700 Human SwissProt: P10599 Human SwissProt: P10639 Mouse SwissProt: P11232 Rat Unigene: 435136 Human Unigene: 260618 Mouse Unigene: 29777 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 硫氧还蛋白(Thioredoxin，Trx)是一类广泛存在于生物体内的多功能酸性蛋白，分子量约12kDa，是一种小分子含硒蛋白质,参与细胞的一系列生化反应，包括酶活性的调节、转录因子的调控等，是重要的酶活力调节蛋白。 硫氧还蛋白的功能绝大多数是依赖于硫氧还蛋白还原靶蛋白中的二硫键。所有硫氧还蛋白均有一个保守的活性中心，Trp-Cys-Gly(Ala)-Pro-Cys，活性中心有两个具有氧还活性的半胱氨酸残基。 |
| 产品图片 |  Sample:Lane 1: Trx-Tagged fusion protein (full length) Overexpression E.coli Lysate (Cat#: bs-33018P) at 4 ug Primary: Anti-Thioredoxin/Trx (bs-0458R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kD Observed band size: 18 kD  Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Thioredoxin) Polyclonal Antibody, Unconjugated (bs-0458R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Thioredoxin) Polyclonal Antibody, Unconjugated (bs-0458R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Thioredoxin) Polyclonal Antibody, Unconjugated (bs-0458R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. |